Fsc-a -

Fsc-a -

To understand FSC-A, one must first understand the phenomenon of Forward Scatter (FSC). When a laser beam intersects a particle (such as a cell) in a fluid stream, the light is diffracted, refracted, and scattered. The light that passes around the cell and continues in the same general direction as the laser is collected by a detector placed directly in line with the laser path. This detector is the Forward Scatter detector.

FSC-A gives you a measurement of cell size – larger cells produce more forward scatter. To understand FSC-A, one must first understand the

Dead or dying cells often exhibit altered light scattering properties. As the cell membrane integrity fails, the cell may shrink or swell, and the internal architecture collapses. This often results in cells that have lower FSC-A than healthy cells. In many experiments, a distinct population of "debris" or dead cells can be gated out using FSC-A, ensuring that subsequent analysis is performed only on viable cells. This detector is the Forward Scatter detector

To understand FSC-A, one must first understand the phenomenon of Forward Scatter (FSC). When a laser beam intersects a particle (such as a cell) in a fluid stream, the light is diffracted, refracted, and scattered. The light that passes around the cell and continues in the same general direction as the laser is collected by a detector placed directly in line with the laser path. This detector is the Forward Scatter detector.

FSC-A gives you a measurement of cell size – larger cells produce more forward scatter.

Dead or dying cells often exhibit altered light scattering properties. As the cell membrane integrity fails, the cell may shrink or swell, and the internal architecture collapses. This often results in cells that have lower FSC-A than healthy cells. In many experiments, a distinct population of "debris" or dead cells can be gated out using FSC-A, ensuring that subsequent analysis is performed only on viable cells.