Highly dependent on specific kickstart or firmware files. How to Install an EmuELEC BIOS Pack
Often performs better or shows the startup animation with gba_bios.bin . emuelec bios pack
Without the BIOS:
If you are on a Mac or Linux, right-click the new bios folder > Properties > Permissions. Ensure "Group" and "Others" have "Read & Write" access. On Windows, this is automatic. Highly dependent on specific kickstart or firmware files